Ngwa iku ume Feline Pentaplex Nucleic Acid Nchọpụta Ngwa

【Ihe ndabere】
Ọrịa feline elu akụkụ okuku ume iku ume (FURD) bụ otu n'ime ihe kacha mkpa na-akpata ọrịa na-anwụ anwụ na nwamba na-eto eto.Ihe mgbaàmà nke ụlọ ọgwụ FURD bụ ahụ ọkụ, agụụ na-ebelata, ịda mbà n'obi, serous, mucous ma ọ bụ purulent secretions na anya na oghere imi, edema ma ọ bụ ọnya na oropharynx, salivation, na ụkwara na imi mgbe ụfọdụ.Ọrịa ndị a na-ahụkarị bụ feline calicivirus (FCV), feline herpesvirus ụdị 1 (FHV-I), Mycoplasma (M. felis), Chlamydia felis (C. felis) na Bordetella bronchiseptica (Bb).

【Ụkpụrụ nke usoro ule】
Feline Respiratory Pathogen Pentaplex Nucleic acid Detection Kit bụ in vitro nucleic acid amplification test maka nucleic acid nke FHV-1, M. felis, FCV, Bordetella bronchiseptica (Bb) na C. felis.
Ihe reagent nke lyophilized nwere ụzọ abụọ izizi, nyocha, ntụgharị transcriptase, DNA polymerase, dNTPs, surfactant, ihe nchekwa na lyoprotectant.
Nnwale a dabere na usoro atọ dị mkpa: (1) nkwadebe nlele akpaaka iji wepụta mkpokọta nucleic acid nke ụdị site na AIMDX 1800VET;(2) ntụgharị ntụgharị nke RNA ebumnuche iji mepụta DNA nkwado (cDNA);(3) Mgbakwunye PCR nke cDNA lekwasịrị anya site na iji primers agbakwunyere, yana nchọpụta n'otu oge nke nyocha TaqMan gbawara agbawa nke na-enye ohere ịchọpụta ngwaahịa ebumpụta ụwa nke ebumnuche.