Canine Cortisol (cCortisol) bụ hormone steroid nke adrenal cortex na-emepụta.Cortisol na-enyere aka ịnọgide na-enwe ọkwa shuga ọbara mgbe niile, na-ebelata mbufụt ma na-enyere ahụ aka ịchịkwa nchekasị.Ọ bụ ezie na a na-akpọ ọnọdụ dị iche iche nke hormone mmanya na-aba n'anya nke ukwuu kpatara Cushing syndrome (CS), ma nkịta na nwamba nwere ike ịta ahụhụ site na CS, bụ nke a na-ahụkarị na nkịta karịa nwamba.Nkịta nke etiti na agadi (ihe dị ka afọ 7 ruo 12)
yikarịrị ka ọ ga-ebute ọrịa ahụ.Ọrịa ahụ na-amalite nwayọọ nwayọọ na mgbaàmà mmalite adịghị mfe ịchọpụta.Enwere ike ịchọpụta CS na ụlọ ọgwụ site na adrenocorticotropic hormone (ACTH) ule mkpali na ule suppression dexamethasone, na ụdị ya dị iche iche: adrenal-dependent (ATH) na pituitary-dependent (PDH).
Ngwaahịa a na-eji fluorescence immunochromatography iji chọpụta ọnụọgụ nke cCortisol na serum/plasma nkịta.Ụkpụrụ bụ isi: A na-akara T na C ahịrị na akpụkpọ ahụ nitrocellulose, a na-ekpuchi eriri T na cCortisol antigen a, a na-efesa mpempe akwụkwọ ahụ na fluorescent nanomaterial labeled antibody b nke nwere ike ịmata cCortisol kpọmkwem.
ọ cCortisol na sample bụ nke mbụ akara na nanomaterial.Ihe mgbochi b na-ejikọta iji mepụta mgbagwoju anya, wee chromatograph na-agbago elu.Mgbagwoju anya na-asọmpi na antigen T-line a enweghị ike ijide ya;N'ụzọ megidere nke ahụ, mgbe enweghị cCortisol na nlele ahụ, mgbochi b na-ejikọta na antigen a.Mgbe a na-agbanye ọkụ na-akpali akpali, ihe nano na-emepụta ihe mgbaàmà fluorescent, na ike nke mgbaàmà na-adabere na ntinye nke cCortisol na nlele.
Ebe ọ bụ na e guzobe ya, anyị factory e ịzụlite mbụ ụwa klas ngwaahịa na adhering ụkpụrụ
nke àgwà mbụ.Ngwaahịa anyị enwetala aha ọma na ụlọ ọrụ na ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị ochie.