Ihe nrịbama Feline Health jikọtara ọnụ (ihe 5-6)

【 Nzube ule】
Feline pancreatic lipase (fPL): Pancreas bụ nke abụọ kasị ukwuu digestive gland n'ime anụ ahụ (nke mbụ bụ imeju), nke dị n'ihu afo nke ahu, kewara n'aka ekpe na aka nri lobes.Isi ọrụ ya bụ izoputa enzymes dị mkpa maka ahụ. A na-ekewa pancreatitis n'ime nnukwu pancreatitis na pancreatitis na-adịghị ala ala.Nbibi nke mbụ kpatara na-abụkarị nwa oge, ebe nke ikpeazụ na-ahapụ fibrosis na-adịgide adịgide na atrophy n'oge mbufụt na-adịghị ala ala ugboro ugboro.N'ime ha, ọrịa pancreatitis na-adịghị ala ala bụ ihe dị ka 2/3 nke nnukwu pancreatitis.
Cholyglycine (CG) bụ otu n'ime conjugated cholic acid nke etolite site na nchikota cholic acid na glycine.Glycocholic acid bụ ihe kachasị mkpa bile acid na serum n'oge ime ime.Mgbe mkpụrụ ndụ imeju mebiri emebi, nnabata nke CG site na mkpụrụ ndụ imeju belatara, na-ebute mmụba nke ọdịnaya CG n'ọbara.Na cholestasis, excretion nke cholic acid site na imeju na-emebi, na ọdịnaya nke CG na-alaghachi na mgbasa ọbara na-abawanye, nke na-emekwa ka ọdịnaya CG dị n'ọbara na-abawanye. A na-echekwa acid bile na gallbladder, nke nwere ike ikpochapụ. site na imeju afọ mgbe nri.N'otu aka ahụ, ọrịa imeju na mgbochi bile duct nwere ike ime ka index na-adịghị mma.
Cystatin C bụ otu n'ime ndị na-edozi ahụ.Ọrụ kachasị mkpa nke anụ ahụ bụ ịhazi ọrụ nke cysteine ​​protease, nke nwere mmetụta mgbochi kachasị ike na cathepsin B, papain, fig protease, na cathepsin H na m wepụtara site na lysosomes.Ọ na-arụ ọrụ dị mkpa na metabolism nke peptides intracellular na protein, karịsịa na metabolism nke collagen, nke nwere ike imebi ụfọdụ prehormones ma hapụ ha n'ime anụ ahụ ezubere iche iji rụọ ọrụ dị iche iche nke ndu ha.Ọkpụkpụ ụbụrụ nke eketa na amyloidosis bụ ọrịa metụtara kpọmkwem mkpụrụ ndụ mkpụrụ ndụ cystatin C, nke nwere ike ibute mgbawa akwara ụbụrụ, ọbara ọgbụgba na nsonaazụ ndị ọzọ dị njọ.Akụrụ bụ naanị ebe ikpochapụ cystatin C na-ekesa, na mmepụta nke cystatin C na-adịgide adịgide.Ọkwa cystatin C serum na-adabere na GFR, nke bụ akara ngosi endogenous kacha mma iji gosipụta mgbanwe nke GFR.Mgbanwe nke ọdịnaya nke mmiri ahụ ndị ọzọ na-ejikọta ya na ọrịa dị iche iche.
NT-proBNP (N-terminal pro-brain natriuretic peptide), nke a makwaara dị ka peptide diuretic B-ụdị, bụ hormone protein nke ezoro ezo site na cardiomyocytes na ventricles nke obi.Mgbe ọbara mgbali ventricular na-abawanye, ventricular dilation, myocardial hypertrophy, ma ọ bụ nrụgide na myocardium na-abawanye, ihe mmalite nke NT-proBNP, proBNP (nke nwere 108 amino acids), na-ezobe n'ime ọbara site na cardiomyocytes.
Mkpokọta cat allergen IgE (fTIgE): IgE bụ ụdị immunoglobulin (Ig) nwere ịdị arọ molekụla nke 188kD yana ọdịnaya dị obere na serum.A na-ejikarị ya eme ihe maka nchọpụta nke mmeghachi omume nfụkasị ahụ.Na mgbakwunye, ọ nwekwara ike inye aka na nyocha nke ọrịa parasitic na multiple myeloma.1. Mmeghachi omume nfụkasị: mgbe mmeghachi omume nfụkasị ahụ na-eme, ọ na-eduga na mmụba nke allergen lgE.Ka ihe nfụkasị ahụ lgE dị elu, ka mmeghachi ahụ nfụkasị ahụ dị njọ karị.2. Ọrịa nje nje: mgbe nje butere anụ ụlọ ahụ, allergen lgE nwekwara ike ịbawanye, nke na-emetụtakarị nrịanrịa dị nro nke proteins parasite na-akpata.Na mgbakwunye, ọnụnọ a na-akọ na ọrịa kansa nwekwara ike itinye aka na mbuli elu nke mkpokọta IgE.
【 Ụkpụrụ nchọpụta】
Ngwaahịa a na-eji fluorescence immunochromatography iji chọpụta ọnụọgụgụ ọdịnaya nke fPL/CG/fCysC/fNT-proBNP/fTIgE n'ime ọbara nwamba.Ụkpụrụ bụ isi bụ na akpụkpọ ahụ nitrocellulose bụ akara T na C, na eriri T na-ekpuchi ya na mgbochi nke na-amata kpọmkwem antigen.A na-efesa ihe mgbochi ahụ na fluorescent nanomaterial ọzọ akpọrọ antibody b nke nwere ike ịmata antigen kpọmkwem.Ihe mgbochi dị na sample na-ejikọta nanomaterial akpọrọ antibody b iji mepụta mgbagwoju anya, nke na-ejikọta ya na T-line antibody A iji mepụta usoro Sanwichi.Mgbe ọkụ mkpali na-enwu, nanomaterial na-ewepụta akara fluorescent.Ejikọtara ike nke mgbaàmà ahụ nke ọma na ntinye antigen dị na nlele ahụ.