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Ngwakọta ọnụ ọgụgụ nke canine Total Thyroxine Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (cTT4)
Nnwale Nzube
T4 bụ isi ngwaahịa nke thyroid secretion, na ọ bụkwa ihe na-adịghị mma akụkụ nke iguzosi ike n'ezi ihe nke hypothalamic-anterior pituitary-thyroid regulatory usoro. Ọ na-abawanye ọnụego metabolic basal ma na-arụ ọrụ dị mkpa na mmepe nke sel anụ ahụ niile. A na-echekwa T4 na follicle thyroid yana njikọ thyroglobulin, na nzuzo ma wepụta ya n'okpuru iwu nke TSH. Ihe karịrị 99% nke T4 na serum dị n'ụdị njikọ na protein ndị ọzọ. Nnwale maka ngụkọta T4 na nlele ọbara nwere ike ịma ma ọ bụrụ na thyroid na-arụ ọrụ nke ọma.
Ụkpụrụ nchọpụta
Ngwaahịa a na-eji fluorescence immunochromatography iji chọpụta ọnụọgụgụ ọdịnaya nke cTT4 na serum/plasma nkịta. Ụkpụrụ bụ isi: A na-akara T na C ahịrị na akpụkpọ ahụ nitrocellulose, a na-ekpuchi eriri T na cTT4 antigen a, a na-efesakwa pad na-ejikọta ya na fluorescent nanomaterial labeled antibody b nke nwere ike ịmata kpọmkwem cTT4. A na-ebu ụzọ kpọ cTT4 dị na nlele ahụ na nanomaterial. Ihe mgbochi b na-ejikọta iji mepụta mgbagwoju anya, wee chromatograph na-agbago elu. Mgbagwoju anya na-asọmpi na antigen T-line a enweghị ike ijide ya; N'ụzọ megidere nke ahụ, mgbe enweghị cTT4 na nlele ahụ, mgbochi b na-ejikọta na antigen a. Mgbe a na-eme ka ọkụ na-akpali akpali, ihe nano na-emepụta ihe mgbaàmà fluorescent, na ike nke mgbaàmà na-adabere na ntinye nke cTT4 na nlele.
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