Ngwa ngwa nke Progesterone Canine (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (cProg)

[Aha ngwaahịa]

Aha: cProg otu nzọụkwụ ule

 

[Nkọwa nkwakọ ngwaahịa]

10 ule/igbe


Nkọwa ngwaahịa

Mkpado ngwaahịa

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Ebumnuche nke nchọpụta

Ntinye uche nke progesterone canine na serum metụtara ogbo nke nkịta estrus.E jiri ya tụnyere LH, ntinye nke cProg na-arị elu mgbe niile n'oge estrus nkịta nwanyị, nke dị mfe iji soro ma nwee ike gbanwee n'oge oge kachasị mma maka ịmụ nwa bụ 3-6 ụbọchị mgbe ọnụ ọgụgụ LH gasịrị, dabere na nwanyị. ọnọdụ estrus nke nkịta.N'etiti ụmụ nwanyị dị iche iche, ọkwa nke progesterone kwekọrọ na oge kachasị mma dịgasị iche iche, n'ozuzu sitere na 0-50ng / ml, ma e nwekwara ihe karịrị nke ahụ Na nso a, ya mere, a na-ejikọta ogo keratinization nke epithelium ikpu. dynamic real-time monitoring of serum progesterone concentrations Usoro nleba anya nwere ike imeziwanye ohere nke echiche nke nkịta nwanyị.

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Ụkpụrụ nchọpụta

Achọpụtara ọdịnaya cProg dị na serum/plasma nkịta site na fluorescence immunochromatography.Ụkpụrụ bụ isi: Fiber nitrate E nwere ahịrị T na C na ihe nkiri ahụ n'otu n'otu, a na-ekpuchikwa eriri T na cProg antigen a, nke nwere ike ịmata cProg kpọmkwem site n'ịfesa na pad.
cProg na sample e bu ụzọ jikọta ya na nanomaterial labeled antibody b iji mepụta mgbagwoju anya, wee ruo n'ogo nke elu, mgbagwoju ahụ na-asọmpi na T-line antigen a enweghị ike ijide;Kama, mgbe enweghị sample N'ihu cProg, antibody b na-ejikọta na antigen a.Mgbe ọkụ ọkụ ọkụ na-akpali akpali, nanomaterial na-ewepụta akara fluorescence.Ike nke mgbaàmà na-adabere na ntinye nke cProg na nlele.

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Nsonaazụ ule

Ebe ọ bụ na ọkwa progesterone kacha mma metụtara ụdị, afọ na nha nke nkịta, ọ nweghị uru zuru oke, usoro ndị a.
Maka ntụnye aka naanị, a na-atụ aro ka ụlọ nyocha ma ọ bụ ụlọ ọgwụ ọ bụla guzobe usoro ntụaka nke ya dịka ụlọọgwụ siri kwuo
Ọ bụghị na okpomọkụ:1ng/ml;
Estrus:ntinye nke progesterone ji nwayọọ nwayọọ na-abawanye, okirikiri ahụ na-abụkarị ụbọchị 7-8;Iji meziwanye ọganihu nke afọ ime, a na-enyocha progesterone mbụ
Ntinye uche kwesịrị ịdị n'ime oke nke 10-50ng / ml, a na-atụ aro ka ịmụba ugboro abụọ.
10-30ng / ml:mbụ mating n'ime 3h, nke abụọ mating n'ime 48h;
30-60ng / ml:mbụ mating n'ime 2h na nke abụọ mating n'ime 24h;
60-80ng/ml:2h maka ịkwa akwa.
Oke nchọpụta nke ngwa a bụ 1-80ng/ml.Ọ bụrụ na ọ gafere oke, kpalite <1ng/ml, ma ọ bụ> 80 ng/ml.


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