Ngwakọta ihe nrịbama ahụike Canine(ihe 5-6)

【 Nzube ule】
Canine pancreatic lipase (cPL): Canine pancreatitis bụ ọrịa infiltrative na-akpali akpali nke pancreas.N'ozuzu, enwere ike kewaa ya na nnukwu pancreatitis na pancreatitis na-adịghị ala ala.Enwere ike ịhụ infiltration neutrophil nke pancreatic, necrosis pancreatic, necrosis peripancreatic abụba, edema na mmerụ ahụ na nnukwu pancreatitis.Enwere ike ịhụ fibrosis pancreatic na atrophy na pancreatitis na-adịghị ala ala.E jiri ya tụnyere nnukwu pancreatitis, pancreatitis na-adịghị ala ala adịghị emerụ ahụ, mana ọ na-adịkarị.Mgbe nkịta na-arịa ọrịa pancreatitis, pancreas na-emebi emebi, ọkwa nke lipase pancreatic na ọbara na-abawanye nke ukwuu.Ka ọ dị ugbu a, lipase pancreatic bụ otu n'ime ihe ngosipụta kachasị mma maka nchọpụta ọrịa pancreatitis na nkịta.
Cholyglycine (CG) bụ otu n'ime conjugated cholic acid nke etolite site na nchikota cholic acid na glycine.Glycocholic acid bụ ihe kachasị mkpa bile acid na serum n'oge ime ime.Mgbe mkpụrụ ndụ imeju mebiri emebi, nnabata nke CG site na mkpụrụ ndụ imeju belatara, na-ebute mmụba nke ọdịnaya CG n'ọbara.Na cholestasis, excretion nke cholic acid site na imeju na-emebi, ọdịnaya CG na-alaghachi na mgbasa ọbara na-abawanye, nke na-abawanye ọdịnaya nke CG n'ime ọbara.
Cystatin C bụ otu n'ime ndị na-edozi ahụ.Ka ọ dị ugbu a, Cys C bụ ihe na-akpata endogenous nke na-emezu ihe achọrọ nke ezigbo akara GFR endogenous.Ọ bụ ihe na-emetụ n'ahụ na nke a kapịrị ọnụ maka ntule ọrụ gbasara akụrụ canine.
N-terminal pro-ụbụrụ natriuretic peptide (Canine NT-proBNP) bụ ihe zoro ezo site na cardiomyocytes na Canine ventricle ma nwee ike iji ya dị ka nchọpụta nchọpụta maka ọdịda obi kwekọrọ.Ntinye uche nke cNT-proBNP n'ime ọbara na-ejikọta na oke ọrịa ahụ.Ya mere, NT-proBNP nwere ike ọ bụghị naanị nyochaa ịdị njọ nke nnukwu obi na-adịghị ala ala, ma a ga-ejikwa ya mee ihe dị ka ihe ngosi nke prognosis ya.
Mkpokọta ihe nfụkasị canine IgE (cTIgE): IgE bụ ụdị immunoglobulin (Ig) nke nwere ibu molekụla nke 188kD yana ọdịnaya dị obere na serum.A na-ejikarị ya eme ihe maka nchọpụta nke mmeghachi omume nfụkasị ahụ.Na mgbakwunye, ọ nwekwara ike inye aka na nyocha nke ọrịa parasitic na multiple myeloma.1. Mmeghachi omume nfụkasị: mgbe mmeghachi omume nfụkasị ahụ na-eme, ọ na-eduga na mmụba nke allergen lgE.Ka ihe nfụkasị ahụ lgE dị elu, ka mmeghachi ahụ nfụkasị ahụ dị njọ karị.2. Ọrịa nje nje: mgbe nje butere anụ ụlọ ahụ, allergen lgE nwekwara ike ịbawanye, nke na-emetụtakarị nrịanrịa dị nro nke proteins parasite na-akpata.Na mgbakwunye, ọnụnọ a na-akọ na ọrịa kansa nwekwara ike itinye aka na mbuli elu nke mkpokọta IgE.

【 Ụkpụrụ nchọpụta】
Ngwaahịa a na-eji fluorescence immunochromatography iji chọpụta ọnụọgụ cPL/CG/cCysC/cNT-proBNP/cTIgE n'ime ọbara canine.Ụkpụrụ bụ isi bụ na akpụkpọ ahụ nitrocellulose bụ akara T na C, na eriri T na-ekpuchi ya na mgbochi nke na-amata kpọmkwem antigen.A na-efesa ihe mgbochi ahụ na fluorescent nanomaterial ọzọ akpọrọ antibody b nke nwere ike ịmata antigen kpọmkwem.Ihe mgbochi dị na sample na-ejikọta nanomaterial akpọrọ antibody b iji mepụta mgbagwoju anya, nke na-ejikọta ya na T-line antibody A iji mepụta usoro Sanwichi.Mgbe ọkụ mkpali na-enwu, nanomaterial na-ewepụta akara fluorescent.Ejikọtara ike nke mgbaàmà ahụ nke ọma na ntinye antigen dị na nlele ahụ.