Feline N-terminal Pro-ụbụrụ Natriuretic Peptide Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals) (fNT-proBNP)

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Aha: feline NT-proBNP ule otu nzọụkwụ

 

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10 ule/igbe


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Izugbe

N'oge nzuzo, protein na-arapara na BNP physiologically arụ ọrụ (77th ruo 108th amino acids) na n-terminal iberibe NT-proBNP (1st ruo 76th amino acids).Mgbe BNP, nke dị 32 amino acid ogologo, na-ezo n'ime ọbara, ọ na-ejikọta ya na ndị na-anabata ya (NPRA na NPRB) ma na-achịkwa ọbara mgbali site na usoro dị iche iche.NT-proBNP, nke bụ 76 amino acid ogologo, enweghị ọrụ ndu, ma n'ihi na ọ nwere ogologo ndụ ọkara ndụ karịa BNP, ọ dabara adaba dị ka ihe nchọpụta nke ọrịa obi dị iche iche.Na nyocha ụlọ ọgwụ nke anụ ụlọ, ntinye ọbara nke NT-proBNP na nkịta dị elu karịa 900 pmol / L, nwamba dị elu karịa 270 pmol / L, na enwere nnukwu ihe ize ndụ nke ọrịa obi.Otú ọ dị, ekwesịrị iburu n'uche na n'ihi na NT-proBNP na-apụ apụ site na akụrụ, mgbe anụ ahụ na-arịa ọrịa akụrụ, ntinye nke NT-proBNP n'ime ahụ ga-abawanye ma ụgha ụgha ga-apụta na ule ahụ.

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Ụkpụrụ nchọpụta

Ngwaahịa a na-anabata fluorescence immunochromatography iji chọpụta ọdịnaya nke fNT-proBNP na serum/plasma ọnụ ọgụgụ.Ụkpụrụ bụ isi: Enwere ahịrị T na C na nitric acid fiber membrane n'otu n'otu, a na-ekpuchikwa eriri T na mgbochi nke na-amata kpọmkwem fNT-proBNP.A na-efesa pad ngwakọta na fluorescent nanomaterial ọzọ akpọrọ antibody b nke nwere ike ịmata FDT-probNP kpọmkwem.N'ime ihe nlele ahụ, FDT-probNP na-ebu ụzọ jikọta ya na nanomaterial akpọrọ antibody b iji mepụta mgbagwoju anya, wee banye na chromatography elu, mgbagwoju ahụ na-ejikọta ya na T-line antibody A iji mepụta usoro Sanwichi.Mgbe mkpali nke irradiation ọkụ, nanomaterial na-ewepụta akara ngosi fluorescence.A na-ejikọta ike nke mgbama ahụ nke ọma na ntinye nke fNT-proBNP na nlele.


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